9.2).Statisticalmodelincludedthefixedeffectsofglycerol(1degreeoffreedom,DF),oil(2DF),time(3DF)andallinteractions. Random effects were experimental runs from each different week (blocks; 2DF) and the residual error. When significant, the means between treatments were compared using the Fisher’s least significant difference (e.g., the option pdiff command LSMEANS). Significance was declared P≤0.05. Orthogonal contrasts were used to assess linear and quadratic relationships between time and the response variable.