The medium contained the following ingredients (g/
1): glucose, 10.0; peptone, 2.5; yeast extract, 2"5; in
1000 ml distilled water. The ingredients were thor oughly mixed and 20 ml portions placed in 100 ml
Erlenmeyer flasks, which were cotton-plugged and
sterilized at 121°C for 15 min. When the flasks
attained room temperature they were inoculated
with the yeasts. The flasks were shaken (200 rpm)
for 48 h at 30°C and the growth used to inoculate
the different fermentation media.