Thiamine (B1), riboflavin (B2), niacin (B3), folic acid (B9)
and pyridoxine (B6) were analyzed by HPLC with Diode
Array and Fluorescent detector. Sample (6 g) was weighed into
a 100 ml flask. The sample was dissolved in 30 ml warm water
followed by the addition of 0.6 M TCA to remove any protein
present in the sample (Woollard and Indyk, 2002). The mixture
was shaken on a mechanical shaker for 15 min and the
resulting mixture was filtered using a 0.45 lm membrane filter
into a HPLC vial. B1, B3 and B9 contents were detected using
Diode Array detector. B2 and B6 contents were detected by
using a Fluorescence detector.