temperature was increased at a rate of 4 °C/min to 230 °C. Finally an isothermic period of 230 °C
for 10 min followed. FAMEs n-hexane (1μL) was injected into the
column using a Varian 8200 CX Autosampler. The injection port and detector were both maintained at 250 °C. Hydrogen, at 45 psi, functioned
as the carrier gas, while nitrogen was employed as the makeup gas.
Varian Star Chromatography Software recorded the chromatograms