Extraction of carotenoids and flavonoids from yellow Thai silk waste was investigated. The total recovery
of 0.7 mg carotenoids and 5.1 mg flavonoids/g dry weight was obtained by ethanol extraction. Differ-
ent methods for extractions of these pigments were carried out using two benign solvents: ethanol
and subcritical water (SW) to determine the extraction efficiency of the solvents in various extraction
conditions. For extraction of carotenoids, ethanol was suitable as extraction solvent and the amount of
carotenoids increased with increasing temperature and extraction time. For flavonoids, SW extraction
was suitable but the amount of flavonoids decreased with increasing SW temperature and extraction
time due to decomposition at such conditions. In addition, the silk extracts were found to have low
IC 50 values (15.6–23.3 g/ml), the concentrations of the silk extracts that exhibit 50% reduction in 2,2-
azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), (ABTS) free radicals, thus indicating high antioxidant
activities.
Extraction of carotenoids and flavonoids from yellow Thai silk waste was investigated. The total recovery
of 0.7 mg carotenoids and 5.1 mg flavonoids/g dry weight was obtained by ethanol extraction. Differ-
ent methods for extractions of these pigments were carried out using two benign solvents: ethanol
and subcritical water (SW) to determine the extraction efficiency of the solvents in various extraction
conditions. For extraction of carotenoids, ethanol was suitable as extraction solvent and the amount of
carotenoids increased with increasing temperature and extraction time. For flavonoids, SW extraction
was suitable but the amount of flavonoids decreased with increasing SW temperature and extraction
time due to decomposition at such conditions. In addition, the silk extracts were found to have low
IC 50 values (15.6–23.3 g/ml), the concentrations of the silk extracts that exhibit 50% reduction in 2,2-
azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), (ABTS) free radicals, thus indicating high antioxidant
activities.
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