Enzymes was able to grade major percentage of gallic acid (1g/L)in first 12 h (65%) and 100% gallic acid was degraded in 24 h.In the control sets no degradation of phenolic compounds was observed. The remarkable removal of COD during degradation of various phenolic compounds was observed (table3).Due to an of PPO enzyme on gallic acid, there was 97.5 (_0.38**)% removal of COD in 24 h.In Gonzalez et al. study,maximum effluent decolorization vslues and COD reduction attained after 7 days of fungal treatment were 73.3 and 61.7% respectively.The present method costs less time for degradation therefore it is more advantageous than previousiypreviously reported methods. Also PPO enzyme is more effective for removal of COD.