The optimum pH was 7.5 with Tris buffer. The highest bioconversion rate was observed at 35 ◦C, although at 40 ◦C
bioconversion activity was virtually lost.Michaelis–Menten type kinetics adequately described the bioconversion system. Increasing biomass
concentration from 10 to 70 gwet cell weight l−1 favored AD final yield, although the specific AD yield slightly decreased.