*** NOTE:
The extracts entrapped in nanoparticles which are larger than 200 nm, can not pass through the 0.2 micron.
Don’t need to filter
Everything must be cleaned. (Sterile distilled water and clean glassware with 70% Alcohol e.g.)
Separate the experiments with another CO2 incubator
Must always clean after finish experiment
The concentrations of unloaded extracts and loaded extracts must be equivalent.