In situ analysis of BslA localization in the floating biofilm. Confocal scanning laser microscopy images of xy sections through a typical pellicle of wild-type strain NRS1473 (3610, sacA::Phy-spank-gfp) after immunofluorescence staining. (A) −0.2 μm, (B) 0 μm, and (C) 0.4 μm into the height of the pellicle. Fluorescence from the GFP within the cells is false-colored green and fluorescence associated with DyLight594, representing immuno-labeled BslA staining, is false-colored red in the merged image. (Scale bar, 5 μm.)