Understanding nano-emulsification processes is of prime importance for the development of nanoparticulate sys- tems. In the formulation of nano-cosmetics, homogeniza- tion appears to be the favored method of preparation of the cosmetic matrix in order to reduce the size of emul- sion. The use of a mixture design approach appears to be a valuable tool in the investigation of real cosmetic matrix systems consisting of multiple ingredients, the proportions of which, in the mixture, are interrelated. Stable nano- emulsions containing an appropriate amount of emulsifiers were successfully formulated. Different physico-chemical properties of nano-emulsions were measured such as size distribution, mobility, electrophoretic mobility, HLB, tur- bidity and melting point. The optimal formulation was cal- culated using a Nemrod software according to these physico-chemical properties. This study indicates the influ- ence of formulation on physico-chemical properties of each nano-emulsion obtained by the mixture design.