PCR-based methods have shown variable sensitivity for detection ofS. stercoralisin fecal samples.
For detec-tion of intestinal nematodes in stool samples by PCR-based techniques, optimization of DNA isolation method is essen-tial, because of existence of large amounts of PCR-inhibitory
substances in fecal samples such as bacterial proteases, nucle-ases, cell debris, bile acids, and so forth, and also the presence of a thick and complex cuticle that covers the worms