Prior to the first dimension, precast IPG strips were rehydrated with 120 μl rehydration buffer (Bio-Rad), 15 μg of proteins and 3 μl of proteome marker for 2-DE (Serva) (16 h, RT).
Prior to the first dimension, precast IPG stripswere rehydrated with 120 μl rehydration buffer (Bio-Rad), 15 μg ofproteins and 3 μl of proteome marker for 2-DE (Serva) (16 h, RT).