In order to conduct FIA procedures, the Agilent 1200 HPLC system was modified by removing the analytical column. The FIA system, shown schematically in Fig. 2, consisted of a quaternary pump with degasser, thermostated column compartment, diode array detector (DAD), and auto sampler. The DAD was operated at 410 nm, and the detector response was recorded on a personal computer with a Chemstation data processor (Ver. B.03.02).