3.1. Molecular weight distribution of b-LG and DH6
Enzymatic hydrolysis of b-LG using trypsin resulted in a strong
shift in the molecular weight profile as presented in Fig. 1. The main
protein fraction b-LG represents the bulk of peptides in the unhydrolysed
sample as displayed by a band of high intensity around
18 kDa in the SDS-PAGE gel and the SEC results. DH3 shows some
bands at about 18 kDa, which can be attributed to residual intact b-
LG. Additionally, many intermediate peptides can be found between
2.5 and 17 kDa with some bands of higher intensity between
6.2 and 8.2 kDa. The peptide distribution determined by SEC confirms
that almost the half of all peptides in DH3 is below 10 kDa.
Strong bands between 6.2 and 8.2 kDa dominate the SDS-PAGE
profile of DH6, in addition to a band at 2.5 kDa. The SEC results