When determining the effects of diet regime on antibody titres against Salmonella, the ELISA assays were carried out
using a single post-challenge sera sample from each animal (diluted 1:4000). All samples from the CTR group and each of
the three diet regime groups were analysed in duplicate wells on the same plate. In addition, to determine the effects of the
diet regimes on the titres of antibodies that cross-reacted with S. Enteritidis, all samples from the CTR, PRE and PRO groups
were analysed in duplicate wells on the same plate.