To simulate intestinal digestion,
the gastric digest (20 mL) was placed into a polyethylene tube
and the dialysis membrane (containing the water-NaHCO3 mixture)
was completely immersed in that digest until reaching pH
5.0. Later, 5 mL of pancreatin (4 g/L) - bile (25 g/L) mixture was
added to the tube, and the incubation continued for 2 h at 37 C
and 90 rpm.