Pomegranate peel, a waste generated from fruit processing industry, is a potential source of active ingredients
such as polyphenols that are known for their antioxidative properties. In this study, optimization of extraction
conditions for bioactive compounds from pomegranate (Punica granatum L.) peels was done to investigate
the effect of solid/solvent ratio (1:10–1:30), incubation time (15–45 min) and temperature (50–
70 °C) on polyphenol extraction using response surface methodology (RSM). The solvent concentration of
60% ethanol was used to extract the phenolic compounds in each experimental run. The experiment was
designed to study the effect of extraction conditions on response variables such as total phenolic content
(TPC), total flavonoids content (TFC), color index, percent reducing sugars and 2,2-Diphenyl-1-picrylhydrazyl
(DPPH) radical scavenging activity in each experiment run. The results of RSM revealed that regression model
fitted significantly at pr0.01. Using optimization technique, solid to solvent ration of 1:30, temperature of
50 °C and time of extraction of 45 min gives highest yield of 68%, total polyphenolic content of 510 mg gallic
acid equivalent/gm, total flavonoids content of 16.40 mg quercetin/gm, color index (ΔE) of 4.07, percent reducing
sugars of 0.18 mg of invert sugar and DPPH radical scavenging activity of 24.54%.
& 2015 Elsevier Ltd. All rights reserved.
Pomegranate peel, a waste generated from fruit processing industry, is a potential source of active ingredientssuch as polyphenols that are known for their antioxidative properties. In this study, optimization of extractionconditions for bioactive compounds from pomegranate (Punica granatum L.) peels was done to investigatethe effect of solid/solvent ratio (1:10–1:30), incubation time (15–45 min) and temperature (50–70 °C) on polyphenol extraction using response surface methodology (RSM). The solvent concentration of60% ethanol was used to extract the phenolic compounds in each experimental run. The experiment wasdesigned to study the effect of extraction conditions on response variables such as total phenolic content(TPC), total flavonoids content (TFC), color index, percent reducing sugars and 2,2-Diphenyl-1-picrylhydrazyl(DPPH) radical scavenging activity in each experiment run. The results of RSM revealed that regression modelfitted significantly at pr0.01. Using optimization technique, solid to solvent ration of 1:30, temperature of50 °C and time of extraction of 45 min gives highest yield of 68%, total polyphenolic content of 510 mg gallicacid equivalent/gm, total flavonoids content of 16.40 mg quercetin/gm, color index (ΔE) of 4.07, percent reducingsugars of 0.18 mg of invert sugar and DPPH radical scavenging activity of 24.54%.& 2015 Elsevier Ltd. All rights reserved.
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