Using primers for the hrcC gene, a
1.08-kb DNA fragment was amplified from wild-type AAC00-1
and the complemented strain, AAC00-1ΔhrcCcomp. However, the
1.08-kb fragment was absent from AAC00-1ΔhrcC and a smaller
0.4-kb fragment was amplified, indicating deletion of the hrcC
gene. As expected, both amplicons were produced with DNA from
AAC00-1ΔhrcCcomp (Fig. 1).