Hot-water pretreatment of rice straw
The water-insoluble fraction of HWP rice straw was
washed twice with distilled water, dried at 80°C for
16 hours, ground to a particle size of approximately
0.5 mm using a laboratory disintegrator (Sansho Industry
Co. Ltd., Osaka, Japan), and then used as the carbon
source for ethanol fermentation. The composition of the
prepared water-insoluble fraction, which was determined
according to the procedure published by the National