Plant growth and disease severity
Plant height, stem width, fresh and dry weight of
shoot (leaves+stems), and fresh and dry weight of
roots were measured after 50 days of transplant. The
shoot (leaves+stems) and root dry weight of two
plants were determined after drying at 75℃ for 48 h
to constant weight. The measurements of two plants
were considered one replicate, and there were three
replications per treatment (n=3).
The two bacterial strains inoculated single or
together were evaluated under greenhouse conditions
for their ability of controlling tomato gray mold disease.
The disease incidence was determined 50 days
after transplanting. Each plant was scored for the
occurrence of disease symptoms according to parameters
set by Hariprasad et al (2011). The biological
control efficacy was calculated using the following
formula: Disease severity=[(A×1)+(B×2)+(C×3)+(D×
4)+(E×5)+(F×6)]/ Number of plant (Hariprasad et al.,
2011).