Expanded polystyrene (EPS) solution (2%) in chloroform was
casted on petri plates to get thin films (0.3 - 0.5 mm). Similar
procedure was used to get films of pure polystyrene (Fluka,
Germany, Mol. Wt. 100,000). Garden soil from Quaid-i-Azam
University, Islamabad, Pakistan, was mixed with manure (1:0.25).
The films (6 x 2.5) were placed in soil contained in an earthen
flower pot at 6 inches depth. 2% glucose solution (400 ml) was
added to the soil to enhance the microbial growth and population.
The films remained buried for eight months (May - November 2006)