The floating fatty layer, corresponding
to the crude lipid body fraction, was removed from the
surface of the supernatant with a spatula and resuspended in buffer
medium containing a non-ionic detergent (1mMTris, pH 7.5, 2mM DTT, 0.5% Triton X-100, approximately 4 ml g1 fatty layer) for
30 min at 4 C. The suspension was mixed vigorously with a vortex
and centrifuged again as above.