Inoculum was raised by transferring two loopfuls of culture from the stock culture to 100 ml of KB broth in a 250 ml Erlenmeyer flask and incubated at room temperature (28 ± 2 C) on a shaker at 150 rpm for 48 h.
Inoculum was raised by transferring two loopfuls of culture from the stock culture to 100 ml of KB brothin a 250 ml Erlenmeyer flask and incubated at room temperature (28 ± 2 C) on a shaker at 150 rpm for 48 h.