Experimental design and feeding
Experimental design: the cattle and
buffaloes were fed of four experimental diets of
KG, EG, EM and RS according to a Latin Square
(4x4) design for each type of animal. Each period
consisted of 10 days adaptation followed by 4 days
collection of faeces, urine and rumen fl uids. The
animals were housed individually in metabolism
cages. Daily feed allowance was restricted to 70 g
dry matter per kg M0·75 to ensure that all food was
eaten. The animals were fed daily at 8:00, 14:00
and 20:00 h. Feed refusals were daily recorded (if
any remain); water was always available.Sample collection: feed samples were
taken in each experimental period. Faeces were
collected and sampled twice a day and kept in a
refrigerator at +4oC until analysis. Prior to analysis,
faeces were thawed, homogenized, sub-sampled
and dried at 60oC and ground to pass a 1- mm
screen. The urine were also collected quantitatively
and preserved by adding 200 ml of H2SO4 10% (pH
< 3) to the collection jar, sampled in 20 ml bottles
and stored at -20oC. The rumen fl uid was sampled
as procedure as experiment 2.
Digestibility of DM, CP, urinary excretion of allantoin (PD) and NH3-N concentrate in rumen
fl uid were measured.
The forage samples were chopped and
dried at 60oC. Dry matter was determined by oven
drying at 105oC overnight. Crude protein was
determined by the Kjeldahl nitrogen analysis as
N*6.25 using Cu as a catalyst. Urinary excretion
of purine derivative analysis by method has been
standardized by IAEA.
Statistical analysis
Data processed through Excel and Minitab
version 15 software.
Experimental design and feedingExperimental design: the cattle andbuffaloes were fed of four experimental diets ofKG, EG, EM and RS according to a Latin Square(4x4) design for each type of animal. Each periodconsisted of 10 days adaptation followed by 4 dayscollection of faeces, urine and rumen fl uids. Theanimals were housed individually in metabolismcages. Daily feed allowance was restricted to 70 gdry matter per kg M0·75 to ensure that all food waseaten. The animals were fed daily at 8:00, 14:00and 20:00 h. Feed refusals were daily recorded (ifany remain); water was always available.Sample collection: feed samples weretaken in each experimental period. Faeces werecollected and sampled twice a day and kept in arefrigerator at +4oC until analysis. Prior to analysis,faeces were thawed, homogenized, sub-sampledand dried at 60oC and ground to pass a 1- mmscreen. The urine were also collected quantitativelyand preserved by adding 200 ml of H2SO4 10% (pH< 3) to the collection jar, sampled in 20 ml bottlesand stored at -20oC. The rumen fl uid was sampledas procedure as experiment 2.Digestibility of DM, CP, urinary excretion of allantoin (PD) and NH3-N concentrate in rumenfl uid were measured.The forage samples were chopped anddried at 60oC. Dry matter was determined by ovendrying at 105oC overnight. Crude protein wasdetermined by the Kjeldahl nitrogen analysis asN*6.25 using Cu as a catalyst. Urinary excretionof purine derivative analysis by method has beenstandardized by IAEA.Statistical analysisData processed through Excel and Minitabversion 15 software.
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