2.5. Free-radical scavenging activity on DPPH
The free-radical scavenging capacity of each extract was evaluated
according to the procedure of Liyana-Pathirana and Shahidi
(2007) with some modifications. Briefly, 100 ll of extract was
added to the freshly prepared 0.1 mM DPPH solution (1.9 ml),
and the mixture was kept at room temperature in a dark room
for 30 min. The absorbance was read at 517 nm relative to the
control (as 100%) and the percentage of scavenging effect was expressed
as [1 (A517 of sample/A517 of control)] 100.