DNA extracts were used as template DNA (ca. 50 μg∙μL-1)
in 50 μL reactions containing 0.2 mM of each of the four
dNTPs, 0.2 μM of primers 27f and 1492r, 1.5 mM
MgCl2 and 1.25 U BioTaq DNA polymerase (Fermentas,
Lithuania) with the appropriate 1x reaction buffer.