In contrast, RT-multiplex PCR with different pairs of specific primers for amplifying different viral genomes in one reaction
tube can detect two or more targets in a single test. In addition, RT-multiplex PCR assay provides simple, rapid, and cost-effective laboratory diagnosis. This study develops a RT-multiplex PCR method to detect Aichi virus, HPeV, EVs, and HBoV in fecal specimenscollected from infants and children with acute gastroenteritis