for direct shoot regeneration, explants from epicotyl, root leaf petiole and cotyldon were cultured on MS medium containing different concentration of vaeious hormones individually or in combination. For epicotyl and root segments, three orientations, viz horizontal, vertical and inverted, were used. The optimum conditions for growth and development of the cultures (25+-2 ํc; 16 h light period by white fluorescent tubes, light intensity 2000 Lux) were maintained in the growth chamber. Explants with proliferated shoot buds, formed under optimal condition, were transferred to elongation medium. The number of elongated shoots (>_1&>_2 cm) was counted after 4 wk of culture.