The formulation of the Ir/C ink used for the working and counter electrodes has been discussed in previous publications. As described, a 787 mm × 584 mm polyester sheet was for the substrate of the sensor on which surface printed silver ink was used as the electrical contact. The biosensor prototype had an overall dimension of 30 mm × 5.5 mm encompassing three electrodes: a Ag/AgCl printed reference electrode, a printed Ir/C working and a Ir/C counter electrodes. The geometric surface area for the printed working electrode was 7.85 × 10−3 cm2. Figure 1 shows the sensor prototype and will be used for ALT detection in this study.
Figure