The resolution between elaidic and oleic acid was critical. The
obtained resolution of trans and cis oleic acid using DB-225ms column
was 1.3 and more than 2 for all other peaks. Analysis on DB-
23 column gave a resolution of 2.9 for elaidic and oleic acid after
optimization. Different retention times and elution orders of
FAMEs on two tested columns gave us confirmation of the identity
of those peaks. Routine samples of pumpkin seed oil and analytical
standards AOCS#1 were analyzed according to the method on both
columns. There was no significant difference between the obtained
results for major and for minor constituents.