2.2. Methods
2.2.1. Preparation of C. papaya leaf extract and 1 mM AgNO3
Fresh leaves of C. papaya (25 g) were diced into fine pieces and transferred to sterile 250 mL conical flask. MilliQ WATER 200 mL was added to the flask and heated at 60 °C for 5–10 min and incubated on sand bath for 30 min to facilitate the formation of aqueous extract. The extract was filtered using Whatman No. 1 filter paper and the filtrate was stored at 4 °C for further use. Silver nitrate (AgNO3, Sigma Aldrich, USA), 0.0421 gm was added to 100 mL of double distilled water and dissolved thoroughly. The solution obtained was transferred to an amber coloured bottle to prevent autoxidation of silver.