RESULTS
In the spectrophotometer, peaks were obtained in two wavelengths: 273 and 345nm. When we fixed the
wavelength at 273nm, we obtained the following absorbance values: 3.0277 (1); 1.5333 (2); 0.7834 (3);
0.4094 (4); 0.2272 (5) and 0.1272 (6). By setting the wavelength at 345 nm, we had the following absorbance
values: 2.5630 (1); 1.2792 (2); 0.6464 (3); 0.3323 (4); 0.1790 (5) and 0.0984 (6). The construction of the
concentration by absorbance graph showed correlations of 0.9999 and 0.9999, respectively. The precision
and accuracy of the assay, as well as the linearity of the calibration curve were determined for intra- and inter-
day on three different days. The precision was expressed as the percent coefficient of variation of each curve.
The statistical data were calculated by ANOVA