Of the 50 strains of S. cerevisiae isolated from Brazilian alcohol
manufacturing plants that were able to grow at 37 C in YNB medium containing 4% glucose (w/v) and 10% ethanol (w/v), S. cerevisiae LBM-1 was chosen for this study because it had the fasted
growth rates in assays conducted at different temperatures. The
strain was identified based on sequence analysis of the D1/D2 region of large subunit rRNA gene. The sequence was 100% identical
to the S. cerevisiae sequence in GenBank