Masset et al. (2012) have suggested the existence of novel pathways for biohydrogen production after they observed the re-consumption of lactate and re-oxidization of formate present in starch fermentation assays by pure stains and co-cultures of Clostridiumspec. A biohydrogen yield ranging from 0.1 upto 2.1 mol/mol of glucose has been generally reported by fermentative bacteria (Fang and Liu, 2002; Morimoto et al., 2004; Maeda et al., 2007) and upto 3 mol/mol glucose by an arsenate reducing Pseudomonas putida (Freikowski et al., 2010).