The initial viscosity of ground chips in water at 32% solid con-tent was 821 m Pa s, which was much lower than that prepared from fresh roots of the same solid content. Cassava chip slurry was brownish in color and could be easily mixed in the system, and thus is easier to hydrolyze and process in industry. Treatment of the cassava chip slurry with 0.05–0.75% (w/w) of the multi-enzyme at pH 5.0, 45◦C for 2 h reduced viscosity by 36.9–78.1% (Fig. 2 b).Target operating viscosity could be achieved by enzymatic treatment with 0.25% (w/w), or greater loadings of multi-enzyme. When multi-enzyme loading was increased to 0.50% and 0.75% (w/w), tar-get viscosity was achieved after shorter treatment times of 60 and 30 min, respectively. As chemical compositions of fresh roots and chips are relatively similar, the results suggest that the differences in the cell wall physical make-up of these two substrates contribute to their differences in viscosity and susceptibility to hydrolytic enzyme attack.