2. Experimental Section
2.1. Animals
The study was conducted on male Wistar rats weighing on average 240 ± 18 g at the beginning of the experiment, supplied by the animal house of the Faculty of Pharmaceutical Sciences, University of São Paulo. The animals were kept in individual cages in a room with controlled temperature of 22 ± 2 °C, on an inverted 12 h light/12 h dark cycle, with lights on at 19:00 h.
The animals had free access to food and water and the consumption of ration and body weight was determined three times a week. The final weight was measured on the day of sacrifice. The animals were followed for a total period of seven weeks, with the first week being devoted to adaptation to the environment and to the diet, and the remaining weeks being used for physical training.
All procedures carried out on the rats were approved by the Ethics Committee on Animal Experimentation of the Faculty of Pharmaceutical Sciences, University of São Paulo, according to the guidelines of the Brazilian College on Animal Experimentation.
- See more at: http://www.mdpi.com/2072-6643/4/11/1767/htm#sthash.gZcSLocm.dpuf
2. Experimental Section2.1. AnimalsThe study was conducted on male Wistar rats weighing on average 240 ± 18 g at the beginning of the experiment, supplied by the animal house of the Faculty of Pharmaceutical Sciences, University of São Paulo. The animals were kept in individual cages in a room with controlled temperature of 22 ± 2 °C, on an inverted 12 h light/12 h dark cycle, with lights on at 19:00 h.The animals had free access to food and water and the consumption of ration and body weight was determined three times a week. The final weight was measured on the day of sacrifice. The animals were followed for a total period of seven weeks, with the first week being devoted to adaptation to the environment and to the diet, and the remaining weeks being used for physical training.All procedures carried out on the rats were approved by the Ethics Committee on Animal Experimentation of the Faculty of Pharmaceutical Sciences, University of São Paulo, according to the guidelines of the Brazilian College on Animal Experimentation.- See more at: http://www.mdpi.com/2072-6643/4/11/1767/htm#sthash.gZcSLocm.dpuf
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2. Experimental Section
2.1. Animals
The study was conducted on male Wistar rats weighing on average 240 ± 18 g at the beginning of the experiment, supplied by the animal house of the Faculty of Pharmaceutical Sciences, University of São Paulo. The animals were kept in individual cages in a room with controlled temperature of 22 ± 2 °C, on an inverted 12 h light/12 h dark cycle, with lights on at 19:00 h.
The animals had free access to food and water and the consumption of ration and body weight was determined three times a week. The final weight was measured on the day of sacrifice. The animals were followed for a total period of seven weeks, with the first week being devoted to adaptation to the environment and to the diet, and the remaining weeks being used for physical training.
All procedures carried out on the rats were approved by the Ethics Committee on Animal Experimentation of the Faculty of Pharmaceutical Sciences, University of São Paulo, according to the guidelines of the Brazilian College on Animal Experimentation.
- See more at: http://www.mdpi.com/2072-6643/4/11/1767/htm#sthash.gZcSLocm.dpuf
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