1. Set up all apparatus required for the variety of blood tests you wish to perform (i.e., blood typing slide setup, and for blood counts: dilution pipettes, diluents, hemocytometer.)
2. Wash hands well with soap and water.
3. Swab a less-used finger (i.e. ring finger on non-writing hand) with 70% EtOH. Lance finger tip with quick firm jab to the side of the fingertip pad, wipe off first drop with clean dry cotton ball. Dispose of the used lancet safely in sharps container.
4. Fill capillary tube to within 1-2 cm of top with blood by slightly tipping it to allow blood to flow down-hill into tube. Avoid bubbles.
5. Holding tube horizontally, press filled end into sealing putty (Crit-o-seal) to plug end. It can then be stored vertically in the Crit-o-seal tray until ready to be centrifuged. (Continue collecting the rest of the samples require for your exercises before clotting occurs.)
6. Lie the tube in the centrifuge Hct head with plugged end to the outside, note the number of your slot. Ensure that a balancing hematocrit tube is placed opposite, either by someone placing their tube there, or by adding an empty tube
7. Securely screw down top of head. Turn on centrifuge to speed 6, run for 5 minutes, turn off.
8. When rotation has stopped, remove tube, note appearance.
1. Set up all apparatus required for the variety of blood tests you wish to perform (i.e., blood typing slide setup, and for blood counts: dilution pipettes, diluents, hemocytometer.)2. Wash hands well with soap and water.3. Swab a less-used finger (i.e. ring finger on non-writing hand) with 70% EtOH. Lance finger tip with quick firm jab to the side of the fingertip pad, wipe off first drop with clean dry cotton ball. Dispose of the used lancet safely in sharps container.4. Fill capillary tube to within 1-2 cm of top with blood by slightly tipping it to allow blood to flow down-hill into tube. Avoid bubbles.5. Holding tube horizontally, press filled end into sealing putty (Crit-o-seal) to plug end. It can then be stored vertically in the Crit-o-seal tray until ready to be centrifuged. (Continue collecting the rest of the samples require for your exercises before clotting occurs.) 6. Lie the tube in the centrifuge Hct head with plugged end to the outside, note the number of your slot. Ensure that a balancing hematocrit tube is placed opposite, either by someone placing their tube there, or by adding an empty tube 7. Securely screw down top of head. Turn on centrifuge to speed 6, run for 5 minutes, turn off. 8. When rotation has stopped, remove tube, note appearance.
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