MATERIALS AND METHODS
Fish and sample preparation
Three hundred male Nile tilapia
(Oreochromis niloticus L.) from the Faculty of
Fisheries, Kamphaeng Saen campus, Kasetsart
University were used in this study. They were
divided into 3 groups (100 fish per group) with an
average weight of 5.7±0.5, 35.8±2.5 and 92.1±5.9