Culture medium contained DMEM containing 4.5 g/l with L-glutamine, without sodium pyruvate (Life Technologies,
Paisley, UK) and supplemented with 10% foetal bovineserum, 100 U/ml penicillin, 100 mg/l streptomycin and geneticin
– G418, 400 lg/ml purchased from Sigma (Dorset, UK).
Culture medium contained DMEM containing 4.5 g/l with L-glutamine, without sodium pyruvate (Life Technologies,Paisley, UK) and supplemented with 10% foetal bovineserum, 100 U/ml penicillin, 100 mg/l streptomycin and geneticin– G418, 400 lg/ml purchased from Sigma (Dorset, UK).
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