The current study showed that a simple and rapid test panel kit could be successfully used to detect SE in eggs in conjunction with various enrichment methods. A novel method for extracting antigen out of egg contents has potential to increase the sensitivity of the test panel kit or other rapid detection systems by avoiding the viscous nature of egg contents and reduce costs of detection of SE by avoiding an enrichment broth. The advantages of the extraction procedure are (1) to provide effective wicking of sample, (2) to generate very clear diagnostic bars (the positive bar) on the strip and (3) to increase sensitivity by avoiding dilution of egg contents in PBS. The developed lateral flow test kit could provide a simple, rapid, and inexpensive method for egg producers and processors to test specifically for Salmonella group D1
serovars, such as SE, in egg samples.