Phenazine methosulfate (PMS) (also known as
N-methylphenazonium methosulfate), L-15 (Leibovitz)
cell culture medium (with L-glutamine) and MEM
(minimal essential medium) cell culture medium (with
Earle’s salt, NEAA and L-glutamine) were purchased from
Himedia Laboratories Pvt. Ltd. (India). XTT {2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)
carbonyl]-2H-tetrazolium hydroxide} was obtained from
Sigma Chemical Co.(St. Louis, MO, USA). Cellular
DNA fragmentation ELISA (Cat. No. 11 585 045 001)
to determine apoptosis was procured from Roche
Diagnostics, Germany. The remaining chemicals and
solvents used were of standard analytical grade and HPLC
grade, respectively