The metabolic potency in terms of short chain fatty acid (SCFA) production of B. pullicaecorum that had been subjected to gastro- intestinal conditions was assessed by further incubation for another 18 h under more optimal growth conditions. Therefore the bottles after stomach (pH 2, pH 3, pH 4 and pH 6) and small in- testine conditions were supplemented with M2GSC medium (50% v/v). As a control, SCFA production of B. pullicaecorum was deter- mined in 24 h incubation in UGIT simulation medium without pepsin, bile salts and pancreatin supplemented with M2GSC me- dium (50% v/v).