Polyploidy induction of plant is a method for reforming plant to selective breeding. In general, polyploid plants have larger leaves and flowers than autopolyploid plants. Using Colchicine treatment under aseptic condition for increasing plant chromosome number to explore its secondary metabolite production has been used as standard protocol. Colchicine, a compound that effectively arrests mitosis at the anaphase stage, has been found to have a significant effect on polyploidy plant induction in vitro. Despite many reports of applying polyploidy techniques to plant by using micro- explant in liquid media MS mixed with 2% (v/v) DMSO, BA 10 μM with 0.09-0.5% (w/v) Colchicine [6]. The present study aimed to evaluate the effects of culture media and explant types on callus and shoot induction of A. muricata L. under aseptic condition for acetogenins secondary metabolite productions.