Invasion was initiated by placing stationary-phase blastospores of C. albicans on the membrane, which stimulated cellular changes in the blastospores. Many of the blastospores developed hyphae with phospholipase activity concentrated at the growing tip. The activity was highest where the hyphae were in direct contact with the membrane (149). In general, only hyphae invaded the membrane successfully. Based on these results, the investigators proposed that extracellular phospholipases were important in the invasion of tissue by C. albicans