The residual was dissolved in 1.5 mL of 0.2 M HCl solution,
and passed through a 0.45 lmmembrane filter. Twenty microlitres
of the hydrolysates were injected using an auto-sampler. Mixed
standard amino acids with taurine standard were analysed before
sampling. The amino acids were identified and quantified by comparing
peak profiles of the squid samples with standard amino acid
profiles. On the basis of the amino acid composition, amino acid
score (AAS), essential amino acid index (EAAI), estimated protein
efficiency ratio (ePER), and biological value (BV) were calculated
according to the reported method for evaluating protein quality
of the squid muscles