In 1993, Belzung and co-authors published nutritional studies comparing the pattern of fat accumulation in Wistar rats fed high fat diets (20% w/w) having n-6/n-3 fatty acids ratios of 6.85/1 (1:1 mixture of beef tallow and olive oil), 0.3/1 (herring oil) and 0.14/1 (a concentrate of EPA (eicosapentaenoic acid = C20:5n-3) and DHA (docosahexaenoic acid = C22:6n-3) (F. Belzung et al. Fish oil n-3 fatty acids selectively limit the hypertrophy of abdominal fat depots in growing rats fed high-fat diets. The American Physiological Society 1993; Rl 111 -Rl 118). They showed that the animals grew the same on all of these three high fat diets and that their subcutaneous and mesenteric adipose tissues were not affected. However, the animals fed herring oil or EPA/DHA concentrate in stead of beef tallow/olive oil, accumulated less abdominal adipose tissue than those fed the mixture of beef tallow and olive oil. The authors speculated that the differences they could observe were related to the highly different metabolism of these three lipid sources, including diet induced differences in the animals' membrane fatty acid composition and differences in fatty acid synthesis, prostaglandin production and modulation of hepatic enzyme activities. It should be noted that omega-3 constituted as much as 40 % (used as concentrate) and 20 % (herring oil) of the total dietary lipid (> 20% w/w) in these studies, corresponding to a daily intake of omega-3 in the range of 0.7 to 1.4 grams. In studies with rats on the effect of EPA or DHA on expression of genes involved in lipid and glucose metabolism in white adipose tissues (T. Raclot et al. Site-specific regulation of gene expression by n-3 polyunsaturated fatty acids in rat white adipose tissues. Journal of Lipid Research 1997;38:1963-1971), the daily intake of these fatty acids was 0.9 grams. In conclusion, Wistar rats consuming very high amounts offish oil or concentrated omega-3, accumulate less visceral fat than rats feeding on a beef tallow/olive oil diet.
 
In 1993, Belzung and co-authors published nutritional studies comparing the pattern of fat accumulation in Wistar rats fed high fat diets (20% w/w) having n-6/n-3 fatty acids ratios of 6.85/1 (1:1 mixture of beef tallow and olive oil), 0.3/1 (herring oil) and 0.14/1 (a concentrate of EPA (eicosapentaenoic acid = C20:5n-3) and DHA (docosahexaenoic acid = C22:6n-3) (F. Belzung et al. Fish oil n-3 fatty acids selectively limit the hypertrophy of abdominal fat depots in growing rats fed high-fat diets. The American Physiological Society 1993; Rl 111 -Rl 118). They showed that the animals grew the same on all of these three high fat diets and that their subcutaneous and mesenteric adipose tissues were not affected. However, the animals fed herring oil or EPA/DHA concentrate in stead of beef tallow/olive oil, accumulated less abdominal adipose tissue than those fed the mixture of beef tallow and olive oil. The authors speculated that the differences they could observe were related to the highly different metabolism of these three lipid sources, including diet induced differences in the animals' membrane fatty acid composition and differences in fatty acid synthesis, prostaglandin production and modulation of hepatic enzyme activities. It should be noted that omega-3 constituted as much as 40 % (used as concentrate) and 20 % (herring oil) of the total dietary lipid (> 20% w/w) in these studies, corresponding to a daily intake of omega-3 in the range of 0.7 to 1.4 grams. In studies with rats on the effect of EPA or DHA on expression of genes involved in lipid and glucose metabolism in white adipose tissues (T. Raclot et al. Site-specific regulation of gene expression by n-3 polyunsaturated fatty acids in rat white adipose tissues. Journal of Lipid Research 1997;38:1963-1971), the daily intake of these fatty acids was 0.9 grams. In conclusion, Wistar rats consuming very high amounts offish oil or concentrated omega-3, accumulate less visceral fat than rats feeding on a beef tallow/olive oil diet.
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