All equipments used for the bioassay study were calibrated and. validated. Sterilized glassware (Class B) such as Petri plates, test. tubes, volumetric flasks, pipettes and sterile borer were used in the. experiment. Steam Sterilizer / Autoclave (Make-Nat steel) was used. to sterilize the media at 121 1C and 15 psi for 15 min. Glycerol stocks of microbial cultures stored at -80 1C Deep freezers (Make. - Haier) were used as test strains. Identification of Levofloxacin was performed by an FTIR spectroscope (Perkin Elmer) and. HPLC (Make-Agilent Technologies) was used for comparative. study. Bioassay plates were incubated at 37 1C inside the incubator. (Make-Thermolab) for bacterial growth. Zones of inhibition were measured by an antibiotic zone reader (Make-Aarachal. Corporation)