Introduction
Several biotechnological strategies involving the
in vitro culture of cells, tissues and plant organs have
been developed and refined as alternatives to
conventional conservation strategies. These
techniques overcome various limitations that are
inherent to conventional ex situ and in situ
conservation methods and allow for the pest-free
exchange of plant genetic resources (Moosikapala
& Te-Chato, 2010; Scherwinski-Pereira & Costa,
2010). Because of the threat of Huanglongbing
(HLB) disease, which is caused by Candidatus
liberibacter spp. and has had devastating effects on the
global citrus industry, the removal of citrus
collections from open field conditions is essential.
Recourses, such as in vitro conservation, are
alternative approaches for establishing backup
collections and may ensure the availability of healthy
material for storage and exchange.