The biogas amount was registered into a log book. The analyses of concern were determined
according to the German DIN-Norm and performed twice weekly: one for the influent and again for the
effluent. The analyses included: Total Solids (TS), Volatile Solids (VS), Chemical Oxygen Demand
(COD), nitrogen (N) and phosphate (PO4); these last three parameters were analyzed as total and soluble
form. Glucose and sucrose were determined spectrophotometrically after enzymatic digestion by a test
kit according to the manufacturer’s instructions.