Healthy Wistar rats weighing between 180 and 200 g
were obtained from the Laboratory Animal House, College
of Health Sciences, Ladoke Akintola University of
Technology (LAUTECH), Ogbomoso, Nigeria. They were
kept in a well-ventilated and hygienic laboratory
environment. The rats were allowed free access to standard
rat feed pellets and clean water ad libitum. All experimental
procedures were conducted in accordance with National
Institute of Health Guide for the Care and Use of
Laboratory Animals [13] as well as Ethical Guidelines for
the Use of Laboratory Animals in LAUTECH, Ogbomoso,
Nigeria.